Human Fibroblast Growth Factor 8

Mfr.No.

E3914Hu

Description

This sandwich kit is for the accurate quantitative detection of human Fibroblast Growth Factor 8 (also known as FGF-8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

AIGF;Androgen-induced growth factor;FGF 8;FGF8;FGF-8;Fibroblast growth factor 8;HBGF-8;Heparin-binding growth factor 8

Reactivity

Human

Detection Range

10-2000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

26525

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

4.94ng/L

Background

Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system (PubMed: 16384934, PubMed: 16597617, PubMed: 8663044). Plays a role in neurite outgrowth in hippocampal cells (PubMed: 21576111).

Gene ID	2253
UniProt ID	P55075
Antigen	FGF8

Research Area

Signal Transduction

Protocol

These standard curves of E3914Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.127	2.201	2.146
	2.275
600ng/L	1.402	1.374	1.319
	1.345
300ng/L	0.857	0.82	0.765
	0.783
150ng/L	0.417	0.443	0.388
	0.469
75ng/L	0.292	0.278	0.224
	0.265
0ng/L	0.062	0.055	0
	0.047

Precision

We measured random samples of E3914Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	399.7	24.35	6.1
Intra-Assay	2	18	824	29.88	3.6
Intra-Assay	3	18	256.6	13.07	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105352	E3914Hu	48T	$320		Add to Cart
BHE12105352	E3914Hu	96T	$458		Add to Cart

Human Fibroblast Growth Factor 8 - FGF-8 ELISA Kit

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