Human Fibroblast Growth Factor-23

Mfr.No.

E0059Hu

Description

This sandwich kit is for the accurate quantitative detection of Fibroblast growth factor-23 (also known as FGF-23) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FGF 23;FGF23;FGF-23;Fibroblast growth factor 23;Fibroblast growth factor 23 C-terminal Peptide;Fibroblast growth factor 23 N-terminal Peptide;Phosphatonin;Tumor-derived hypophosphatemia-inducing factor

Reactivity

Human

Detection Range

5-1500pg/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

27954

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.49pg/ml

Background

Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL (By similarity). Acts directly on the parathyroid to decrease PTH secretion (By similarity). Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization.

Gene ID	8074
UniProt ID	Q9GZV9
Antigen	FGF23

Research Area

Signal Transduction

Protocol

These standard curves of E0059Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800pg/ml	2.23	2.112	2.07
	1.994
400pg/ml	1.334	1.405	1.364
	1.477
200pg/ml	0.703	0.705	0.663
	0.707
100pg/ml	0.416	0.424	0.382
	0.431
50pg/ml	0.16	0.156	0.115
	0.152
0pg/ml	0.056	0.042	0
	0.027

Precision

We measured random samples of E0059Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	284.3	14.43	5.1
Intra-Assay	2	18	345.5	12.54	3.6
Intra-Assay	3	18	164.3	9.42	5.7

Storage	2-8°C

Reference	Relationships Between Left Ventricular Structure and Function According to Cardiac MRI and Cardiac Biomarkers in End-Stage Renal Disease Journal: Can J Cardiol. 2017 Apr;33(4):501-507. Serum sTWEAK and FGF-23 Levels in Hemodialysis and Renal Transplant Patients Journal: Int J Organ Transplant Med. 2017;8(2):110-116. Serum sTWEAK and FGF-23 Levels in Hemodialysis and Renal Transplant Patients Journal: Int J Organ Transplant Med. 2017;8(2):110-116.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101794	E0059Hu	96T	$458		Add to Cart
BHE12101794	E0059Hu	48T	$320		Add to Cart

Human Fibroblast Growth Factor-23 - FGF-23 ELISA Kit

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