Human Fibroblast Growth Factor-21

Mfr.No.

E1983Hu

Sensitivity

3.52pg/ml

Detection Range

7-1500pg/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Fibroblast Growth Factor-21 (also known as FGF-21) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FGF 21;FGF21;FGF-21;Fibroblast growth factor 21

Molecular Weight

22300

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E1983Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	209.7	8.55	4.1
Intra-Assay	2	18	507	26.25	5.2
Intra-Assay	3	18	679.3	20.68	3

Background

Stimulates glucose uptake in differentiated adipocytes via the induction of glucose transporter SLC2A1/GLUT1 [removed]but not SLC2A4/GLUT4 expression). Activity requires the presence of KLB.

Protocol

These standard curves of E1983Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800pg/ml	1.739	1.812	1.765
	1.884
400pg/ml	0.929	0.978	0.931
	1.028
200pg/ml	0.461	0.451	0.404
	0.441
100pg/ml	0.204	0.213	0.166
	0.222
50pg/ml	0.056	0.061	0.014
	0.066
0pg/ml	0.034	0.047	0
	0.061

Gene ID	26291
UniProt ID	Q9NSA1

Research Area	Signal Transduction

Storage	2-8°C

Reference	Serum level of fibroblast growth factor 21 in type 2 diabetic patients with and without metabolic syndrome Journal: Acta Endocrinol (Buchar). Jul-Sep 2016;12(3):257-261

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103544	E1983Hu	48T	$320		Add to Cart
BHE12103544	E1983Hu	96T	$458		Add to Cart

Human Fibroblast Growth Factor-21 - FGF-21 ELISA Kit

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