Human FGF19 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1160

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.029	0.098	0.157	0.258	0.574	1.194	1.73	2.067

Description

Human FGF19 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human FGF19 in cell culture supeatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Alternative Names

Fibroblast growth factor 19;FGF-19;FGF19;UNQ334/PRO533;

Molecular Weight

24003 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: L25-K216

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FGF19

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Tissue homogenates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	103	396	669	152	388	709
Standard deviation	6.69	17	25.4	10.94	20.95	34.74
CV (%)	6.5%	4.3%	3.8%	7.2%	5.4%	4.9%

Protein Function

Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. .

Background

FGF19, Fibroblast growth factor 19, is a protein that in humans is encoded by the FGF19 gene. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. The FGF19 gene is mapped to 11q13.3. The deduced 216-amino acid FGF19 protein contains asignal sequence and 2 cysteine residues that are conserved in the FGF family. Expression of this gene was detected only in fetal but not adult brain tissue. Synergistic interaction of the chick homolog and Wnt-8c has been shown to be required for initiation of inner ear development. FGF19 stimulates hepatic protein and glycogen synthesis but does not induce lipogenesis. The effects of FGF19 are independent of the activity of either insulin or the protein kinase Akt and, instead, are mediated through a mitogen-activated protein kinase signaling pathway that activates components of the protein translation machinery and stimulates glycogen synthase activity.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	9965
Gene Name	FGF19
UniProt ID	O95750
Protein Name	Fibroblast growth factor 19
Tissue Specificity	Expressed in fetal brain, cartilage, retina, and adult gall bladder. .

Applications	ELISA
Research Area	Cancer,Growth Factors,Growth Factors/Hormones,Neurogenesis,Neurology Process,Neuroscience,Secreted,Signal Transduction,Signaling Pathways,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000023	EZ1160	5 plates/kit	$500		Add to Cart

Human FGF19 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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