Human Fetuin A/AHSG ELISA Kit PicoKine®

Mfr.No.

EK0757

Sensitivity

<10 pg/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.071	0.306	0.396	0.673	1.169	1.585	1.719	2.026

Description

Human Fetuin A / AHSG ELISA Kit PicoKine® (96 Tests). Quantitate Human AHSG in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Alpha-2-HS-glycoprotein;Alpha-2-Z-globulin;Ba-alpha-2-glycoprotein;Fetuin-A;Alpha-2-HS-glycoprotein chain A;Alpha-2-HS-glycoprotein chain B;AHSG;FETUA;PRO2743;

Molecular Weight

39325 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A19-V367

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Fetuin A

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1646	4628	22095	1708	5021	21195
Standard deviation	126.74	185.82	883.8	143.47	2244	1229.31
CV (%)	7.7%	4%	4%	8.4%	4.5%	5.8%

Protein Function

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions.

Background

Alpha2-HS glycoprotein (AHSG), also known as fetuin-A, is a plasma protein displaying high-affinity interaction with calcium phosphate, by which ectopic vascular calcification is prevented. The AHSG polymorphism is attributable to the hereditary variation of AHSG and phosphate serum levels, which may affect skeletal development and chronic disorders such as vascular calcification.1Human plasma protein alpha 2-HS-glycoprotein (AHSG) is composed of two polypeptide chains, A and B, encoded by a single mRNA. Southe blot analysis of mouse x human somatic cell hybrids has mapped the AHSG gene to human chromosome 3 in the region 3q21----qter. Using a recombinant plasmid containing a 1,538 bp insert spanning the entire AHSG coding region, AHSG was localized to chromosomal bands 3q27----q29 by in situ hybridization.2

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1646	1726	1766	1528	1666	90.88	5.4%
Sample 2	4628	4495	4603	4388	4528	95.27	2.1%
Sample 3	22095	25102	22613	25109	23729	1387.88	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	197
Gene Name	AHSG
UniProt ID	P02765
Protein Name	Alpha-2-HS-glycoprotein
Tissue Specificity	Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins.
Sequence	Belongs to the fetuin family.

Applications	ELISA
Research Area	Cardiovascular,Metabolism,Neurogenesis,Neurology Process,Neuroscience,Obesity,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000607	EK0757	96 wells/kit	$499		Add to Cart

Human Fetuin A/AHSG ELISA Kit PicoKine®

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