Human FCRL5 ELISA Kit PicoKine®

Mfr.No.

EK1935

Sensitivity

<0.1 ng/ml

Detection Range

0.78 ng/ml - 50 ng/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.012	0.109	0.141	0.258	0.593	1.007	1.144	1.880

Description

Human FCRL5 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCRL5 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

FcR-like protein 5; FcRL5; BXMAS1; Fc receptor homolog 5; FcRH5; Immune receptor translocation-associated protein 2; CD307e; FCRH5; IRTA2; UNQ503/PRO820

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q16-R844

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FCRL5

Subcellular Localization

Cell membrane, Single-pass type I membrane protein

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1090	6877	21168	1262	6894	22254
Standard deviation	56.68	433.25	1016.06	61.84	510.16	1179.46
CV (%)	5.2%	6.3%	4.8%	4.9%	7.4%	5.3%

Protein Function

May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells.

Background

Fc receptor-like protein 5 is a protein that in humans is encoded by the FCRL5 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and the Fc-receptor like family. This gene and several other Fc receptor-like gene members are clustered on the long arm of chromosome 1. The encoded protein is a single-pass type I membrane protein and contains 8 immunoglobulin-like C2-type domains. This gene is implicated in B cell development and lymphomagenesis. Alteatively spliced transcript variants encoding different isoforms have been identified.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1090	1154	1262	1129	1158	63.82	5.5%
Sample 2	6877	7371	6834	7977	7264	462.19	6.3%
Sample 3	21168	22254	20007	18723	20538	1315.08	6.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	83416
Gene Name	FCRL5
UniProt ID	Q96RD9
Tissue Specificity	Expressed in marginal zone B-cells, immunoblasts, tonsillar germinal center centrocytes and in the intraepithelial and interfollicular regions of the tonsil. Expressed in many lymphoma cell lines and on hairy cell leukemia cells. Isoform 1, isoform 3, isoform 4 and isoform 5 are detected in lymph node, spleen, bone marrow, and small intestine with preponderance of isoform 3. Expressed in mature and memory B-cells and down-regulated in germinal center cells.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001442	EK1935	96 wells/kit	$499		Add to Cart

Human FCRL5 ELISA Kit PicoKine®

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