Human FCRL1 ELISA Kit PicoKine®

Mfr.No.

EK1930

Sensitivity

<20 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.017	0.074	0.127	0.226	0.480	0.822	1.297	2.024

Description

Human FCRL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human FCRL1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fc receptor-like protein 1; FcR-like protein 1; FcRL1; Fc receptor homolog 1; FcRH1; IFGP family protein 1; Hifgp1; Immune receptor translocation-associated protein 5; CD307a; FCRL1; FCRH1; IFGP1; IRTA5

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-S307

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FCRL1

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	42	182	837	51	193	947
Standard deviation	2.18	9.65	46.04	3.11	12.35	63.45
CV (%)	5.2%	5.3%	5.5%	6.1%	6.4%	6.7%

Protein Function

May function as an activating coreceptor in B-cells. May function in B-cells activation and differentiation.

Background

Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alteative splicing results in multiple transcript variants.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	42	40	42	45	42	1.78	4.2%
Sample 2	182	167	169	161	169	7.66	4.5%
Sample 3	837	797	842	900	844	36.73	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	115350
Gene Name	FCRL1
UniProt ID	Q96LA6
Tissue Specificity	Primarily expressed in secondary lymphoid tissues by mature subsets of B-cells. Detected in spleen, lymph node, heart, skeletal muscle, kidney, liver and placenta. Specifically expressed by mature B lineage cells with higher expression in naive versus memory B-cells.

Applications	ELISA
Research Area	Immunoglobulins,Immunology,Receptors

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001514	EK1930	96 wells/kit	$750		Add to Cart

Human FCRL1 ELISA Kit PicoKine®

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