Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine®

Mfr.No.

EK1312

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.007	0.124	0.199	0.255	0.541	0.813	1.408	2.266

Description

Human Fc gamma RIIB/C (CD32b/c) (FCGR2B/C) ELISA Kit PicoKine® (96 Tests). Quantitate Human FCGR2B/C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Low affinity immunoglobulin gamma Fc region receptor II-c;IgG Fc receptor II-c;CDw32;Fc-gamma RII-c;Fc-gamma-RIIc;FcRII-c;CD32;FCGR2C;CD32, FCG2, IGFR2;FCGR2B

Molecular Weight

35578 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A46-P217

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FCGR2B/C

Subcellular Localization

Isoform IIC4: Cytoplasm .

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	122	404	1372	133	365	1478
Standard deviation	8.05	19.79	97.41	9.7	22.26	130.06
CV (%)	6.6%	4.9%	7.1%	7.3%	6.1%	8.8%

Protein Function

Receptor for the Fc region of complexed immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells.

Background

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	122	125	105	106	114	9.06	7.9%
Sample 2	404	361	357	357	369	19.84	5.3%
Sample 3	1372	1213	1353	1369	1326	66.06	4.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2213, 9103
Antigen	CD32b/c
Gene Name	FCGR2B
UniProt ID	P31994, P31995
Protein Name	Low affinity immunoglobulin gamma Fc region receptor II-b/c
Tissue Specificity	Isoform IIC1 is detected in monocytes, macrophages, polymorphonuclear cells and natural killer cells.
Sequence	Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Applications	ELISA
Research Area	Hematopoietic Progenitors,Immunoglobulins,Immunology,Lymphoid,Myeloid,Receptors,Stem Cells,T Lymphocytic Lineage

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000902	EK1312	96 wells/kit	$750		Add to Cart

Human Fc gamma RIIB/C (CD32b/c)(FCGR2B/C) ELISA Kit PicoKine®

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