Human Far Upstream Element-binding Protein 2

Mfr.No.

E5274Hu

Alternative Names

Far upstream element-binding protein 2;FUSE-binding protein 2;KH type-splicing regulatory protein;KHSRP;KSRP;p75

Reactivity

Human

Detection Range

20-4800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

73115

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

12.87ng/L

Background

Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.

Gene ID	8570
UniProt ID	Q92945
Antigen	KHSRP

Research Area

Others

Protocol

These standard curves of E5274Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2560ng/L	2.334	2.257	2.197
	2.181
1280ng/L	1.499	1.53	1.47
	1.561
640ng/L	0.728	0.744	0.683
	0.759
320ng/L	0.529	0.491	0.431
	0.452
160ng/L	0.276	0.278	0.218
	0.28
0ng/L	0.061	0.06	0
	0.06

Precision

We measured random samples of E5274Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1241.3	45.49	3.7
Intra-Assay	2	18	706.8	33.02	4.7
Intra-Assay	3	18	378.6	19.69	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106637	E5274Hu	48T	$320		Add to Cart
BHE12106637	E5274Hu	96T	$458		Add to Cart

Human Far Upstream Element-binding Protein 2 - KHSRP ELISA Kit

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