Human FABP2/I-FABP ELISA Kit PicoKine®

Mfr.No.

EK1410

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.099	0.196	0.285	0.325	0.466	0.672	1.160	1.923

Description

Human FABP2/I-FABP ELISA Kit PicoKine® (96 Tests). Quantitate Human FABP2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Fatty acid-binding protein, intestinal;Fatty acid-binding protein 2;Intestinal-type fatty acid-binding protein;I-FABP;FABP2;FABPI;

Molecular Weight

15207 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-D132

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human FABP2`

Subcellular Localization

Cytoplasm.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	49	270	1038	51	272	977
Standard deviation	2.69	14.31	61.24	3.57	18.76	74.25
CV (%)	5.5%	5.3%	5.9%	7%	6.9%	7.6%

Protein Function

FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long- chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor.

Background

Fatty acid-binding protein 2 (FABP2), also known as Intestinal-type fatty acid-binding protein (I-FABP) is a protein that in humans is encoded by the FABP2 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family with nearly twenty identified members. FABPs are divided into at least three distinct types, namely the hepatic-, intestinal- and cardiac-type. Using a human cDNA probe for in situ hybridization studies, they regionalized the assignment to 4q28-q31. The intestinal form of FABP was mapped to mouse chromosome 3 between the amylase and alcohol dehydrogenase-3 loci. Also, they may be responsible in the modulation of cell growth and proliferation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	49	56	59	50	53	4.15	7.8%
Sample 2	270	308	313	281	293	18.01	6.1%
Sample 3	1038	997	1031	1019	1021	15.56	1.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	2169
Antigen	FABP2/I-FABP
Gene Name	FABP2
UniProt ID	P12104
Protein Name	Fatty acid-binding protein, intestinal
Tissue Specificity	Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. .

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001148	EK1410	96 wells/kit	$499		Add to Cart

Human FABP2/I-FABP ELISA Kit PicoKine®

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