Human F-Box Only Protein 32 - FBXO32 ELISA Kit

Mfr.No.

E3293Hu

Sensitivity

1.57ng/L

Detection Range

3-900ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human F-box Only Protein 32 (also known as FBXO32) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Atrogin-1;F-box only protein 32;FBXO 32;FBXO32;MAFbx;Muscle atrophy F-box protein

Molecular Weight

41637

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3293Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	259.3	7.33	2.8
Intra-Assay	2	18	112.3	7.16	6.4
Intra-Assay	3	18	170.8	9.18	5.4

Background

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.

Protocol

These standard curves of E3293Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.296	2.362	2.3
	2.428
240ng/L	1.27	1.282	1.22
	1.294
120ng/L	0.81	0.817	0.755
	0.825
60ng/L	0.457	0.482	0.42
	0.507
30ng/L	0.233	0.269	0.207
	0.305
0ng/L	0.075	0.062	0
	0.049

Gene ID	114907
UniProt ID	Q969P5

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104822	E3293Hu	48T	$320		Add to Cart
BHE12104822	E3293Hu	96T	$458		Add to Cart

Human F-Box Only Protein 32 - FBXO32 ELISA Kit

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