Matrices listed below were spiked with certain level of EREG and the recovery rates were calculated by comparing the measured value to the expected amount of EREG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-98
92
EDTA plasma(n=5)
88-104
98
heparin plasma(n=5)
93-105
100
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EREG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
EREG ELISA Kit allows for the in vitro quantitative determination of EREG concentrations in serum, plasma, tissue homogenates and other biological fluids.