Matrices listed below were spiked with certain level of EPX and the recovery rates were calculated by comparing the measured value to the expected amount of EPX in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
92
EDTA plasma(n=5)
86-104
93
heparin plasma(n=5)
85-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of EPX and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
EPX ELISA Kit allows for the in vitro quantitative determination of EPX concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human EPX(Eosinophil peroxidase) ELISA Kit