Human Epithelial Neutrophil Activating Peptide 78

Mfr.No.

E0131Hu

Description

This sandwich kit is for the accurate quantitative detection of human Epithelial Neutrophil Activating Peptide 78 (also known as ENA-78) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-X-C motif chemokine 5;CXCL 5;CXCL5;ENA-78(1-78;ENA-78(8-78;ENA-78(9-78;Epithelial-derived neutrophil-activating protein 78;Neutrophil-activating Peptide ENA-78;Small-inducible cytokine B5;

Reactivity

Human

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11972

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.16ng/mL

Background

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

Gene ID	6374
UniProt ID	P42830
Antigen	CXCL5

Research Area

Immunology

Protocol

These standard curves of E0131Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	2.219	2.178	2.142
	2.138
24ng/mL	1.324	1.293	1.257
	1.263
12ng/mL	0.684	0.621	0.584
	0.558
6ng/mL	0.302	0.336	0.3
	0.371
3ng/mL	0.162	0.175	0.138
	0.187
0ng/mL	0.042	0.037	0
	0.032

Precision

We measured random samples of E0131Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.4	0.66	5.3
Intra-Assay	2	18	19.1	0.93	4.9
Intra-Assay	3	18	24.2	0.77	3.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101866	E0131Hu	96T	$458		Add to Cart
BHE12101866	E0131Hu	48T	$320		Add to Cart

Human Epithelial Neutrophil Activating Peptide 78 - ENA-78 ELISA Kit

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