Human Epidermal Growth Factor

Mfr.No.

E0144Hu

Description

This sandwich kit is for the accurate quantitative detection of human Epidermal Growth Factor (also known as EGF) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

EGF;Epidermal growth factor;Pro-epidermal growth factor;Urogastrone

Reactivity

Human

Detection Range

0.5-200ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

133994

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.28ng/L

Background

EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium Channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed: 10964941).

Gene ID	1950
UniProt ID	P01133
Antigen	EGF

Research Area

Signal Transduction; Cell Biology; Cancer

Protocol

These standard curves of E0144Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/L	2.223	2.204	2.164
	2.185
60ng/L	1.336	1.356	1.316
	1.377
30ng/L	0.814	0.829	0.789
	0.845
15ng/L	0.541	0.503	0.463
	0.464
7.5ng/L	0.305	0.287	0.247
	0.269
0ng/L	0.045	0.04	0
	0.035

Precision

We measured random samples of E0144Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	54.6	1.9	3.5
Intra-Assay	2	18	20.7	1.59	7.7
Intra-Assay	3	18	50	3.59	7.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101879	E0144Hu	96T	$458		Add to Cart
BHE12101879	E0144Hu	48T	$320		Add to Cart

Human Epidermal Growth Factor - EGF ELISA Kit

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