Human Eosinophil Peroxidase

Mfr.No.

E3442Hu

Description

This sandwich kit is for the accurate quantitative detection of human Eosinophil Peroxidase (also known as EPO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Eosinophil peroxidase;Eosinophil peroxidase heavy chain;Eosinophil peroxidase light chain;EPO;EPX

Reactivity

Human

Detection Range

0.2-60mU/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

81040

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.12mU/ml

Background

Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis.

Gene ID	8288
UniProt ID	P11678
Antigen	EPX

Research Area

Cancer

Protocol

These standard curves of E3442Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40mU/ml	2.142	2.158	2.129
	2.175
20mU/ml	1.299	1.369	1.34
	1.439
10mU/ml	0.635	0.679	0.65
	0.724
5mU/ml	0.394	0.414	0.385
	0.435
2.5mU/ml	0.118	0.16	0.13
	0.201
0mU/ml	0.026	0.03	0
	0.033

Precision

We measured random samples of E3442Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.3	0.46	5.6
Intra-Assay	2	18	28.3	1.15	4.1
Intra-Assay	3	18	16	0.95	5.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104964	E3442Hu	48T	$320		Add to Cart
BHE12104964	E3442Hu	96T	$458		Add to Cart

Human Eosinophil Peroxidase - EPO ELISA Kit

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