Human eosinophil chemotactic factor,ECF ELISA Kit

Human eosinophil chemotactic factor,ECF ELISA Kit

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Mfr.No.

CSB-E04533h

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chemokine (C-C motif) ligand 11, MGC22554, SCYA11, eosinophil chemotactic protein, eotaxin-1, small inducible cytokine A11, small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)

Recovery

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ?2TF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-100
1:2	Average %	87
	Range %	83-91
1:4	Average %	97
	Range %	92-110
1:8	Average %	90
	Range %	86-94

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
80	2.915	2.952	2.934	2.769
40	2.210	2.076	2.143	1.978
20	1.561	1.604	1.583	1.418
10	1.051	1.039	1.045	0.880
5	0.616	0.624	0.620	0.455
2.5	0.396	0.369	0.383	0.218
1.25	0.274	0.279	0.277	0.112
0	0.162	0.167	0.165

Species

Homo sapiens

Sample Type

Serum, Cell culture supernatants, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P51671

Applications	ELISA
Research Area	CCL11

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10502250	CSB-E04533h	96T	$595	Add to Cart
BHE10502250	CSB-E04533h	5x96T	$2856	Add to Cart
BHE10502250	CSB-E04533h	10x96T	$5652.50	Add to Cart

The recovery of human ?2TF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Serum (n=5)