Human Engulfment and Cell Motility Protein 1

Mfr.No.

E4764Hu

Sensitivity

10.48ng/L

Detection Range

20-3800ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Engulfment and Cell Motility Protein 1 (also known as ELMO1) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ELMO 1;ELMO1;Engulfment and cell motility protein 1;Protein ced-12 homolog

Molecular Weight

83829

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4764Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	388.5	13.59	3.5
Intra-Assay	2	18	1883	62.34	3.3
Intra-Assay	3	18	861.5	41.44	4.8

Background

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in association with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1.

Protocol

These standard curves of E4764Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.664	1.733	1.699
	1.802
1000ng/L	0.94	0.946	0.911
	0.951
500ng/L	0.545	0.513	0.479
	0.482
250ng/L	0.193	0.166	0.131
	0.139
125ng/L	0.076	0.107	0.072
	0.138
0ng/L	0.038	0.035	0
	0.031

Gene ID	9844
UniProt ID	Q92556

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106051	E4764Hu	48T	$320		Add to Cart
BHE12106051	E4764Hu	96T	$458		Add to Cart

Human Engulfment and Cell Motility Protein 1 - ELMO1 ELISA Kit

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