Human Endothelin ELISA Kit PicoKine®

Mfr.No.

EK0945

Sensitivity

<0.5 pg/ml

Detection Range

3.9 pg/ml - 250 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	3.9	7.8	15.6	31.2	62.5	125	250
O.D.	0.001	0.039	0.064	0.149	0.301	0.680	1.150	1.821

Description

Human Endothelin ELISA Kit PicoKine® (96 Tests). Quantitate Human Endothelin in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Endothelin-1;Preproendothelin-1;PPET1;Endothelin-1;ET-1;Big endothelin-1;EDN1;

Molecular Weight

24425 MW

Immunogen

A peptide reflecting the target protein in human. Sequence: C53-W73

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Endothelin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	11	51	131	11	51	130
Standard deviation	0.62	2.75	8.64	0.62	2.95	10.92
CV (%)	5.7%	5.4%	6.6%	5.7%	5.8%	8.4%

Protein Function

Endothelins are endothelium-derived vasoconstrictor peptides.

Background

The endothelins is a family of structurally and pharmacologically distinct peptides, which has been identified and sequenced in humans. Three isoforms of human endothelin have been identified: endothelin-1, -2, and -3. Endothelin-1 is a potent, 21-amino acid vasoconstrictor peptide produced by vascular endothelial cells. Endothelins are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. Endothelins are implicated in vascular diseases of several organ systems, including the heart, general circulation and brain. Endothelins are proteins that constrict blood vessels and raise blood pressure. They are normally kept in balance by other mechanisms, but when they are over-expressed, they contribute to high blood pressure (hypertension) and heart disease.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	11	13	12	14	12	1.11	9.2%
Sample 2	51	49	47	47	48	1.65	3.4%
Sample 3	131	117	110	124	120	7.82	6.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1906, 1907, 1908
Gene Name	EDN1
UniProt ID	P05305, P20800, P14138
Protein Name	Endothelin-1, Endothelin-2, Endothelin-3
Tissue Specificity	Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. .
Sequence	Belongs to the endothelin/sarafotoxin family.

Applications	ELISA
Research Area	Cancer,Cancer Metabolism,Cardiovascular,Growth Factors/Hormones,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Signal Transduction,Vasculature

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000717	EK0945	96 wells/kit	$499		Add to Cart

Human Endothelin ELISA Kit PicoKine®

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