Human Endothelin-1 Receptor

Mfr.No.

E3157Hu

Sensitivity

16.03ng/L

Detection Range

30-7000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Endothelin-1 Receptor（also known as EDNRA）in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

EDNRA;Endothelin receptor type A;Endothelin-1 receptor;ET-A;ETA-R;hET-AR

Molecular Weight

48722

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3157Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1446.5	49.86	3.4
Intra-Assay	2	18	2793.6	83.36	3
Intra-Assay	3	18	1140.3	60.88	5.3

Background

Receptor for endothelin-1. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of binding affinities for ET-A is: ET1 ; ET2 ;; ET3.

Protocol

These standard curves of E3157Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	1.862	1.951	1.92
	2.04
2000ng/L	1.165	1.132	1.1
	1.098
1000ng/L	0.617	0.542	0.511
	0.468
500ng/L	0.235	0.234	0.202
	0.233
250ng/L	0.116	0.112	0.08
	0.108
0ng/L	0.025	0.032	0
	0.039

Gene ID	1909
UniProt ID	P25101

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104688	E3157Hu	48T	$320		Add to Cart
BHE12104688	E3157Hu	96T	$458		Add to Cart

Human Endothelin-1 Receptor - EDNRA ELISA Kit

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