Human Endostatin ELISA Kit PicoKine®

Mfr.No.

EK0886

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.068	0.294	0.596	0.980	1.323	1.555	1.695	1.916

Description

Human Endostatin ELISA Kit PicoKine® (96 Tests). Quantitate Human COL18A1 in cell culture supeatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Collagen alpha-1 (XVIII) chain;Endostatin;COL18A1;

Molecular Weight

178188 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H1154-S1335

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Endostatin

Subcellular Localization

Secreted, extracellular space, extracellular matrix .

Sample Type

Cell culture supeatants, Serum, Plasma, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	247	1523	5144	251	1462	5243
Standard deviation	14.07	109.65	216.04	14.3	130.11	319.82
CV (%)	5.7%	7.2%	4.2%	5.7%	8.9%	6.1%

Protein Function

COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube.

Background

Endostatin is a naturally-occurring 20-kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	247	238	232	204	230	16.06	6.9%
Sample 2	1523	1309	1362	1479	1418	86.25	6%
Sample 3	5144	5141	5851	5778	5478	336.99	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	80781
Gene Name	COL18A1
UniProt ID	P39060
Protein Name	Collagen alpha-1(XVIII) chain
Tissue Specificity	Present in multiple organs with highest levels in liver, lung and kidney.
Sequence	Belongs to the multiplexin collagen family.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Collagen,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Invasion/Microenvironment,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000678	EK0886	96 wells/kit	$499		Add to Cart

Human Endostatin ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Endostatin ELISA Kit PicoKine®

Rate it now..!!