Human Endoglycan/PODXL2 ELISA Kit PicoKine®

Mfr.No.

EK1928

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.079	0.137	0.191	0.328	0.567	0.973	1.612	2.313

Description

Human Endoglycan/PODXL2 ELISA Kit PicoKine® (96 Tests). Quantitate Human PODXL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Podocalyxin-like protein 2; Endoglycan; PODXL2; UNQ1861/PRO3742

Immunogen

Expression system for standard: NS0; Immunogen sequence: G33-T500

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Endoglycan/PODXL2

Subcellular Localization

Membrane, Single-pass type I membrane protein

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	205	1739	5795	210	1893	5983
Standard deviation	8.82	109.56	283.96	9.24	119.26	442.74
CV (%)	4.3%	6.3%	4.9%	4.4%	6.3%	7.4%

Protein Function

Acts as a ligand for vascular selectins. Mediates rapid rolling of leukocytes over vascular surfaces through high affinity divalent cation-dependent interactions with E-, P- and L-selectins.

Background

Podocalyxin like 2 is a protein that in humans is encoded by the PODXL2 gene. It is mapped to 3q21.3. This gene is a member of the CD34 family of cell surface transmembrane proteins, which are characterized by an N-terminal extracellular mucin domain, globular and stalk domains, a single pass transmembrane region, and a charged cytoplasmic tail. The encoded protein is a ligand for vascular selectins.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	205	237	210	211	215	12.47	5.8%
Sample 2	1739	1950	1893	1973	1888	91.23	4.8%
Sample 3	5795	6024	5325	5519	5665	265.84	4.6%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	50512
Antigen	Endoglycan/PODXL2
Gene Name	PODXL2
UniProt ID	Q9NZ53
Tissue Specificity	Expressed in T-cells, B-cells and monocytes. Expression is higher on memory and germinal center cells than on naive B-cells (at protein level). Highly expressed in brain. Moderately expressed in pancreas, kidney and lymphoid node. Weakly expressed in liver. Detected in both endothelial cells and CD34+ bone marrow cells.

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Immunology,Innate Immunity,Non-CD,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001440	EK1928	96 wells/kit	$750		Add to Cart

Human Endoglycan/PODXL2 ELISA Kit PicoKine®

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