Human Elongin A - ELOA ELISA Kit

Mfr.No.

E1482Hu

Alternative Names

EloA;Elongin 110 kDa subunit;Elongin-A;RNA polymerase II transcription factor SIII subunit A1;SIII;SIII p110;TCEB3;TCEB3A;Transcription elongation factor B polypeptide 3;

Reactivity

Human

Detection Range

0.3-90ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

89909

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.14ng/mL

Background

SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).

Gene ID	6924
UniProt ID	Q14241
Antigen	EloA

Research Area

Others

Protocol

These standard curves of E1482Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
48ng/mL	1.895	1.918	1.864
	1.94
24ng/mL	1.078	1.043	0.989
	1.007
12ng/mL	0.501	0.548	0.494
	0.594
6ng/mL	0.278	0.29	0.236
	0.301
3ng/mL	0.101	0.132	0.079
	0.164
0ng/mL	0.046	0.054	0
	0.062

Precision

We measured random samples of E1482Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	41.5	1.38	3.32
Intra-Assay	2	18	26.54	1.04	3.93
Intra-Assay	3	18	16.48	0.82	4.95

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103099	E1482Hu	48T	$320		Add to Cart
BHE12103099	E1482Hu	96T	$458		Add to Cart

Human Elongin A - ELOA ELISA Kit

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