Human Elafin/PI3 ELISA Kit PicoKine®

Mfr.No.

EK1117

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.084	0.156	0.204	0.319	0.419	0.628	1.014	1.819

Description

Human Elafin/PI3 ELISA Kit PicoKine® (96 Tests). Quantitate Human PI3 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Elafin;Elastase-specific inhibitor;ESI;Peptidase inhibitor 3;PI-3;Protease inhibitor WAP3;Skin-derived antileukoproteinase;SKALP;WAP four-disulfide core domain protein 14;PI3;WAP3, WFDC14;

Molecular Weight

12270 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A23-Q117

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Elafin

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	64	263	893	70	273	891
Standard deviation	4.22	12.36	51.79	4.9	15.56	61.47
CV (%)	6.6%	4.7%	5.8%	7%	5.7%	6.9%

Protein Function

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Background

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	64	53	57	58	58	3.93	6.7%
Sample 2	263	250	260	236	252	10.54	4.1%
Sample 3	893	966	957	1042	964	52.86	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5266
Gene Name	PI3
UniProt ID	P19957
Protein Name	Elafin

Applications	ELISA
Research Area	Cell Biology,Cell Cycle,Immunology,Innate Immunity,Kinases/Phosphatases,Lipid Signaling,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000771	EK1117	96 wells/kit	$499		Add to Cart

Human Elafin/PI3 ELISA Kit PicoKine®

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