Human Elafin/PI3 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

Mfr.No.

EZ1117

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.01	0.073	0.148	0.271	0.575	0.966	1.789	2.233

Description

Human Elafin/PI3 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human PI3 in cell culture supeatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

Alternative Names

Elafin;Elastase-specific inhibitor;ESI;Peptidase inhibitor 3;PI-3;Protease inhibitor WAP3;Skin-derived antileukoproteinase;SKALP;WAP four-disulfide core domain protein 14;PI3;WAP3, WFDC14;

Molecular Weight

12270 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: A23-Q117

Kit Components

Description
Capture Antibody
Detection Antibody
Avidin-Biotin-Peroxidase Complex (ABC)
Lyophilized Standard

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Elafin/PI3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Tissue homogenates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	225	657	1334	230	645	1742
Standard deviation	12.6	32.2	57.36	17.02	39.99	113.23
CV (%)	5.6%	4.9%	4.3%	7.4%	6.2%	6.5%

Protein Function

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Background

Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoproteinase (SKALP) is a protein that in humans is encoded by the PI3 gene. It is mapped to 20q13.12. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. This gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines. This gene is the neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.

Notes

Material Required But Not Provided:
1. Microplate reader in standard size.
2. Automated plate washer.
3. Incubator.
4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
5. Clean tubes and Eppendorf tubes.
6. 96 well microplate
7. Plate Sealers.
8. Capture Antibody Diluent: PBS.
9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
10. Substrate Solution: Tetramethylbenzidine
11. Stop Solution: 2 N H2SO4
12. Wash Buffer . Recipes:
PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
*Item 6 12 are included in the EZ Set Accessory Kit

Gene ID	5266
Gene Name	PI3
UniProt ID	P19957
Protein Name	Elafin

Applications	ELISA
Research Area	Cell Biology,Cell Cycle,Immunology,Innate Immunity,Kinases/Phosphatases,Lipid Signaling,Protein Phosphorylation,Ser/Thr Kinases,Signal Transduction,Signaling Pathway,TLR Signaling

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000022	EZ1117	5 plates/kit	$500		Add to Cart

Human Elafin/PI3 ELISA Kit EZ-Set^TM (DIY Antibody Pairs)

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