Human ECM1 ELISA Kit PicoKine®

Mfr.No.

EK2283

Sensitivity

<20 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.036	0.095	0.244	0.487	0.652	0.943	1.365	2.058

Description

Human ECM1 ELISA Kit PicoKine® (96 Tests). Quantitate Human ECM1 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Molecular Weight

61 kDa

Immunogen

Expression system for standard: NS0; Immunogen sequence: A20-E540

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	251	1041	3816	180	1013	4617
Standard deviation	18.9	32.79	140.81	13.75	27.76	211.92
CV(%)	7.5%	3.2%	3.7%	7.6%	2.7%	4.6%

Background

Extracellular matrix protein 1 is a protein that in humans is encoded by the ECM1 gene. This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alteatively spliced transcript variants encoding distinct isoforms have been described for this gene.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	251	301	306	301	290	17.65	6.1%
Sample 2	1041	946	960	1121	1017	40.99	4%
Sample 3	3816	2746	4780	2745	3522	215.88	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene Name	ECM1
UniProt ID	Q16610
Protein Name	Extracellular matrix protein 1

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001832	EK2283	96 wells/kit	$499		Add to Cart

Human ECM1 ELISA Kit PicoKine®

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