Human E3 Ubiquitin Protein Ligase Rnf167

Mfr.No.

E3470Hu

Description

This sandwich kit is for the accurate quantitative detection of human E3 Ubiquitin Protein Ligase RNF167 (also known as RNF167) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

E3 ubiquitin-protein ligase RNF167;RING finger protein 167;RING105;RING-type E3 ubiquitin transferase RNF167;RNF 167;RNF167

Reactivity

Human

Detection Range

0.05-15ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

38299

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/ml

Background

May act as an E3 ubiquitin-protein ligase, or as part of the E3 complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2E1, and then transfers it to substrates, such as SLC22A18. May play a role in growth regulation involved in G1/S transition.

Gene ID	26001
UniProt ID	Q9H6Y7
Antigen	RNF167

Research Area

Others

Protocol

These standard curves of E3470Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.233	2.144	2.09
	2.055
4ng/ml	1.614	1.674	1.621
	1.735
2ng/ml	1.093	1.131	1.077
	1.17
1ng/ml	0.703	0.717	0.663
	0.73
0.5ng/ml	0.415	0.432	0.378
	0.449
0ng/ml	0.048	0.054	0
	0.059

Precision

We measured random samples of E3470Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.7	0.19	5
Intra-Assay	2	18	0.7	0.04	5.4
Intra-Assay	3	18	2	0.12	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104976	E3470Hu	48T	$320		Add to Cart
BHE12104976	E3470Hu	96T	$458		Add to Cart

Human E3 Ubiquitin Protein Ligase Rnf167 - RNF167 ELISA Kit

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