Human E3 Ubiquitin-protein Ligase Hace1

Mfr.No.

E5212Hu

Alternative Names

E3 ubiquitin-protein ligase HACE1;HACE1;HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1;HECT-type E3 ubiquitin transferase HACE1;SPPRS

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

102342

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.57ng/L

Background

HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1 is a protein that in humans is encoded by the HACE1 gene.

Gene ID	57531
UniProt ID	Q8IYU2
Antigen	HACE1

Research Area

Cell Biology

Protocol

These standard curves of E5212Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.133	2.152	2.119
	2.171
800ng/L	1.536	1.555	1.522
	1.574
400ng/L	0.767	0.762	0.73
	0.758
200ng/L	0.432	0.407	0.374
	0.381
100ng/L	0.117	0.126	0.094
	0.135
0ng/L	0.041	0.033	0
	0.025

Precision

We measured random samples of E5212Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	543.66	25.52	4.69
Intra-Assay	2	18	1024.87	56.34	5.5
Intra-Assay	3	18	288.62	12.16	4.21

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106575	E5212Hu	48T	$320		Add to Cart
BHE12106575	E5212Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Hace1 - HACE1 ELISA Kit

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