Human E3 Ubiquitin-protein Ligase Chip

Mfr.No.

E5211Hu

Alternative Names

Antigen NY-CO-7;Carboxy terminus of Hsp70-interacting protein;CHIP;CLL-associated antigen KW-8;E3 ubiquitin-protein ligase CHIP;HSPABP2;NY-CO-7;RING-type E3 ubiquitin transferase CHIP;SCA48;SCAR16;STIP1 homology and U box-containing protein 1;STUB1;UBOX1;

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34856

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.46ng/L

Background

STUB1 (STIP1 homology and U-Box containing protein 1) is a human gene that codes for the protein CHIP (C terminus of HSC70-Interacting Protein).

Gene ID	10273
UniProt ID	Q9UNE7
Antigen	STUB1

Research Area

Signal Transduction

Protocol

These standard curves of E5211Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.284	2.235	2.18
	2.187
800ng/L	1.571	1.659	1.603
	1.746
400ng/L	1.103	1.124	1.068
	1.144
200ng/L	0.689	0.692	0.637
	0.696
100ng/L	0.4	0.431	0.376
	0.463
0ng/L	0.05	0.055	0
	0.061

Precision

We measured random samples of E5211Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	836.16	54.29	6.49
Intra-Assay	2	18	273.31	13.38	4.89
Intra-Assay	3	18	206.15	15.86	7.69

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106574	E5211Hu	48T	$320		Add to Cart
BHE12106574	E5211Hu	96T	$458		Add to Cart

Human E3 Ubiquitin-protein Ligase Chip - STUB1 ELISA Kit

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