Human Dysferlin - DYSF ELISA Kit

Mfr.No.

E5208Hu

Alternative Names

DYSF;Dysferlin;Dystrophy-associated fer-1-like protein;FER1L1;Fer-1-like protein 1;LGMD2B;LGMDR2;MMD1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

237295

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.88ng/L

Background

Key calcium ion sensor involved in the Ca2+-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).

Gene ID	8291
UniProt ID	O75923
Antigen	DYSF

Research Area

Cell Biology

Protocol

These standard curves of E5208Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.249	2.215	2.16
	2.18
1200ng/L	1.363	1.292	1.237
	1.221
600ng/L	0.839	0.861	0.806
	0.883
300ng/L	0.434	0.473	0.418
	0.511
150ng/L	0.327	0.31	0.255
	0.293
0ng/L	0.047	0.055	0
	0.063

Precision

We measured random samples of E5208Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1384.75	44.1	3.18
Intra-Assay	2	18	254.85	13.69	5.37
Intra-Assay	3	18	982.57	58.78	5.98

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106571	E5208Hu	48T	$320		Add to Cart
BHE12106571	E5208Hu	96T	$458		Add to Cart

Human Dysferlin - DYSF ELISA Kit

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