Human Dynein Heavy Chain 5 - Axonemal

Mfr.No.

E3543Hu

Sensitivity

0.027ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Dynein Heavy Chain 5,Axonemal (also known as DNAH5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Axonemal beta dynein heavy chain 5;Ciliary dynein heavy chain 5;DNAH 5;DNAH5;Dynein heavy chain 5, axonemal

Molecular Weight

529021

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3543Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	14.3	0.44	3.1
Intra-Assay	2	18	4.9	0.17	3.4
Intra-Assay	3	18	9.5	0.44	4.6

Background

Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Required for structural and functional integrity of the cilia of ependymal cells lining the brain ventricles.

Protocol

These standard curves of E3543Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	1.926	1.834	1.787
	1.743
8ng/ml	1.102	1.016	0.969
	0.93
4ng/ml	0.462	0.478	0.43
	0.493
2ng/ml	0.297	0.22	0.173
	0.143
1ng/ml	0.099	0.105	0.057
	0.11
0ng/ml	0.06	0.047	0
	0.034

Gene ID	1767
UniProt ID	Q8TE73

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105034	E3543Hu	48T	$320		Add to Cart
BHE12105034	E3543Hu	96T	$458		Add to Cart

Human Dynein Heavy Chain 5 - Axonemal - DNAH5 ELISA Kit

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