Human Dynein Heavy Chain 12 - Axonemal

Mfr.No.

E5206Hu

Alternative Names

Axonemal beta dynein heavy chain 12;Axonemal dynein heavy chain 12-like protein;Axonemal dynein heavy chain 7-like protein;Ciliary dynein heavy chain 12;DNAH 12;DNAH12;Dynein heavy chain 12, axonemal;Dynein heavy chain 7-like, axonemal;Dynein heavy chain domain-containing protein 2;

Reactivity

Human

Detection Range

7-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

356942

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.11ng/L

Background

Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity; the force-producing power stroke is thought to occur on release of ADP. Involved in sperm motility; implicated in sperm flagellar assembly (By similarity).

Gene ID	201625
UniProt ID	Q6ZR08
Antigen	DNAH12

Research Area

Others

Protocol

These standard curves of E5206Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.026	1.976	1.92
	1.926
400ng/L	0.969	1.027	0.971
	1.085
200ng/L	0.555	0.544	0.488
	0.534
100ng/L	0.178	0.184	0.128
	0.19
50ng/L	0.105	0.124	0.068
	0.142
0ng/L	0.056	0.056	0
	0.056

Precision

We measured random samples of E5206Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	255.9	12.61	4.9
Intra-Assay	2	18	482.4	13.82	2.9
Intra-Assay	3	18	450.7	19.91	4.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106569	E5206Hu	48T	$320		Add to Cart
BHE12106569	E5206Hu	96T	$458		Add to Cart

Human Dynein Heavy Chain 12 - Axonemal - DNAH12 ELISA Kit

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