Human Dolichol Kinase - DOLK ELISA Kit

Mfr.No.

E2411Hu

Sensitivity

0.022ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Dolichol kinase;DOLK;Transmembrane protein 15

Molecular Weight

59268

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2411Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	8.2	0.27	3.3
Intra-Assay	2	18	4.8	0.3	6.2
Intra-Assay	3	18	2.2	0.1	4.6

Background

Involved in the synthesis of the sugar donor Dol-P-Man which is required in the synthesis of N-linked and O-linked oligosaccharides and for that of GPI anchors.

Protocol

These standard curves of E2411Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	1.879	1.771	1.745
	1.662
4ng/ml	1.054	0.985	0.96
	0.917
2ng/ml	0.523	0.576	0.551
	0.63
1ng/ml	0.167	0.154	0.129
	0.142
0.5ng/ml	0.099	0.081	0.055
	0.062
0ng/ml	0.027	0.025	0
	0.023

Gene ID	22845
UniProt ID	Q9UPQ8

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103949	E2411Hu	48T	$320		Add to Cart
BHE12103949	E2411Hu	96T	$458		Add to Cart

Human Dolichol Kinase - DOLK ELISA Kit

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