Human DNA-binding Protein Inhibitor Id-4

Mfr.No.

E5200Hu

Sensitivity

5.14ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

bHLHb27;Class B basic helix-loop-helix protein 27;DNA-binding protein inhibitor ID-4;ID4;IDB4;Inhibitor of differentiation 4;Inhibitor of DNA binding 4

Molecular Weight

16622

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5200Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	831.86	42.08	5.06
Intra-Assay	2	18	424	18.5	4.36
Intra-Assay	3	18	247.09	10.06	4.07

Background

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation (By similarity).

Protocol

These standard curves of E5200Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	2.154	2.14	2.096
	2.125
600ng/L	1.482	1.52	1.477
	1.559
300ng/L	0.712	0.678	0.634
	0.643
150ng/L	0.455	0.427	0.383
	0.399
75ng/L	0.131	0.128	0.084
	0.126
0ng/L	0.059	0.044	0
	0.028

Gene ID	3400
UniProt ID	P47928

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106563	E5200Hu	48T	$320		Add to Cart
BHE12106563	E5200Hu	96T	$458		Add to Cart

Human DNA-binding Protein Inhibitor Id-4 - ID4 ELISA Kit

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