Human DLL1 ELISA Kit PicoKine®

Mfr.No.

EK1276

Sensitivity

<10 pg/ml

Detection Range

78 pg/ml - 5,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.003	0.232	0.373	0.705	1.012	1.459	1.811	1.902

Description

Human DLL1 ELISA Kit PicoKine® (96 Tests). Quantitate Human DLL1 in cell culture supeatants, serum, plasma (heparin, EDTA) , urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Delta-like protein 1;Drosophila Delta homolog 1;Delta1;H-Delta-1;DLL1;UNQ146/PRO172;

Molecular Weight

78056 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: S22-G540

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human DLL1

Subcellular Localization

Membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	105	634	2353	102	662	2503
Standard deviation	4.62	27.26	94.12	4.69	31.11	137.66
CV (%)	4.4%	4.3%	4%	4.6%	4.7%	5.5%

Protein Function

Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor. .

Background

DLL1 (DELTA-LIKE 1), also known as DL1 or DELTA1, is a protein that in humans is encoded by the DLL1 gene. DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis, and it is also important in cell-to-cell communication. This gene is mapped to chromosome 6q27, near a locus for type I diabetes. Functional analysis suggested that a soluble fusion protein containing the DSL domain of DLL1 and its adjacent 50 N-terminal amino acids increased the viability of hemopoietic cells but inhibited cell death. It has been found that DLL1 blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	105	104	94	106	102	4.81	4.7%
Sample 2	634	578	595	596	600	20.48	3.4%
Sample 3	2353	2202	2459	2342	2339	91.34	3.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	28514
Gene Name	DLL1
UniProt ID	O00548
Protein Name	Delta-like protein 1
Tissue Specificity	Expressed in heart and pancreas, with lower expression in brain and muscle and almost no expression in placenta, lung, liver and kidney.
Sequence	Contains 1 DSL domain.

Applications	ELISA
Research Area	Developmental Biology,Embryogenesis,Embryonic Stem Cells,Epigenetics and Nuclear Signaling,Hematopoietic Progenitors,Intracellular,Neurogenesis,Neurology Process,Neuroscience,Signaling Pathways,Stem Cells,Surface Molecules,Transcription

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000866	EK1276	96 wells/kit	$499		Add to Cart

Human DLL1 ELISA Kit PicoKine®

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