Human Disintegrin and Metalloproteinase Domain-containing Protein 28

Mfr.No.

E5187Hu

Alternative Names

ADAM 28;ADAM28;Disintegrin and metalloproteinase domain-containing protein 28;eMDC II;Epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II;MDC-L;Metalloproteinase-like, disintegrin-like, and cysteine-rich protein L

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

87148

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.018ng/ml

Background

May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation.

Gene ID	10863
UniProt ID	Q9UKQ2
Antigen	ADAM28

Research Area

Others

Protocol

These standard curves of E5187Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.431	2.263	2.186
	2.094
6ng/ml	1.291	1.251	1.175
	1.211
3ng/ml	0.874	0.79	0.714
	0.707
1.5ng/ml	0.536	0.484	0.408
	0.433
0.75ng/ml	0.256	0.263	0.186
	0.27
0ng/ml	0.075	0.076	0
	0.078

Precision

We measured random samples of E5187Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.9	0.12	6.1
Intra-Assay	2	18	8.1	0.23	2.9
Intra-Assay	3	18	5.3	0.32	6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106550	E5187Hu	48T	$320		Add to Cart
BHE12106550	E5187Hu	96T	$458		Add to Cart

Human Disintegrin and Metalloproteinase Domain-containing Protein 28 - ADAM28 ELISA Kit

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