Human Dihydrofolate Reductase

Mfr.No.

E6651Hu

Sensitivity

0.092ng/ml

Detection Range

0.2-70ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

DHFR;DHFRP1;Dihydrofolate reductase;DYR

Molecular Weight

21453

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E6651Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	19.49	0.76	3.9
Intra-Assay	2	18	36.82	1.03	2.8
Intra-Assay	3	18	10.5	0.51	4.88

Background

Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2.

Protocol

These standard curves of E6651Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.027	1.949	1.911
	1.871
20ng/ml	1.014	0.996	0.958
	0.978
10ng/ml	0.441	0.504	0.466
	0.566
5ng/ml	0.277	0.247	0.209
	0.218
2.5ng/ml	0.124	0.123	0.086
	0.122
0ng/ml	0.041	0.038	0
	0.035

Gene ID	1719
UniProt ID	P00374

Research Area	Signal Transduction; Cell Biology; Metabolism

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115311	E6651Hu	48T	$320		Add to Cart
BHE12115311	E6651Hu	96T	$458		Add to Cart

Human Dihydrofolate Reductase - DHFR ELISA Kit

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