Human Dermatopontin/DPT ELISA Kit PicoKine®

Mfr.No.

EK1633

Sensitivity

<10 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.022	0.058	0.117	0.266	0.591	1.157	1.685	2.062

Description

Human Dermatopontin/DPT ELISA Kit PicoKine® (96 Tests). Quantitate Human DPT in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Dermatopontin; Tyrosine-rich acidic matrix protein; TRAMP; DPT

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-V201

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human DPT

Subcellular Localization

Secreted, extracellular space, extracellular matrix.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	100	977	2868	91	933	3012
Standard deviation	7.1	46.89	169.21	7.91	58.77	234.93
CV (%)	7.1%	4.8%	5.9%	8.7%	6.3%	7.8%

Protein Function

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity). .

Background

Dermatopontin?is a?protein?that in humans is encoded by the?DPT?gene. Its an?extracellular matrix?protein with possible functions in cell-matrix interactions and matrix assembly. The protein is found in various tissues and many of its tyrosine residues are sulphated. Dermatopontin is postulated to modify the behavior of?TGF-beta?through interaction with?decorin.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	100	105	105	109	104	3.19	3%
Sample 2	977	1016	907	886	946	52.39	5.5%
Sample 3	2868	2711	2472	2675	2681	141.05	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1805
Gene Name	DPT
UniProt ID	Q07507
Tissue Specificity	Expressed in fibroblasts, heart, skeletal muscle, brain and pancreas. Expressed at an intermediate level in lung and kidney, and at a low level in liver and placenta. Expressed at a lower level in fibroblasts from hypertrophic scar lesional skin and in fibroblasts from patients with systemic sclerosis than in normal skin fibroblasts.

Applications	ELISA
Research Area	Biochemicals,Chemical Type,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001343	EK1633	96 wells/kit	$499		Add to Cart

Human Dermatopontin/DPT ELISA Kit PicoKine®

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