Human Dermato-Pontin - DPT ELISA Kit

Mfr.No.

E1433Hu

Description

This sandwich kit is for the accurate quantitative detection of human Dermatopontin (also known as DPT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Dermatopontin;DPT;TRAMP;Tyrosine-rich acidic matrix protein

Reactivity

Human

Detection Range

0.1-40ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

24005

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.059ng/ml

Background

Seems to mediate adhesion by cell surface integrin binding. May serve as a communication link between the dermal fibroblast cell surface and its extracellular matrix environment. Enhances TGFB1 activity. Inhibits cell proliferation. Accelerates collagen fibril formation, and stabilizes collagen fibrils against low-temperature dissociation (By similarity).

Gene ID	1805
UniProt ID	Q07507
Antigen	DPT

Research Area

Signal Transduction

Protocol

These standard curves of E1433Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.907	1.934	1.885
	1.96
12ng/ml	1.059	1.061	1.012
	1.064
6ng/ml	0.646	0.595	0.547
	0.545
3ng/ml	0.266	0.204	0.155
	0.141
1.5ng/ml	0.063	0.1	0.052
	0.138
0ng/ml	0.05	0.049	0
	0.047

Precision

We measured random samples of E1433Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.7	0.49	4.6
Intra-Assay	2	18	15.6	0.44	2.8
Intra-Assay	3	18	6.3	0.2	3.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103055	E1433Hu	48T	$320		Add to Cart
BHE12103055	E1433Hu	96T	$458		Add to Cart

Human Dermato-Pontin - DPT ELISA Kit

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