Human Deoxyribonuclease-2-alpha

Mfr.No.

E1103Hu

Description

This sandwich kit is for the accurate quantitative detection of human Deoxyribonuclease-2-alpha (also known as DNASE2A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Acid DNase;Deoxyribonuclease II alpha;Deoxyribonuclease-2-alpha;DNase II alpha;DNASE2A;DNL;DNL2;Lysosomal DNase II;R31240_2;

Reactivity

Human

Detection Range

0.1-35ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

39581

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.048ng/ml

Background

Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells.

Gene ID	1777
UniProt ID	O00115
Antigen	DNASE2A

Research Area

Cell Biology

Protocol

These standard curves of E1103Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.084	2.162	2.124
	2.239
10ng/ml	1.649	1.586	1.549
	1.524
5ng/ml	1.19	1.191	1.154
	1.192
2.5ng/ml	0.733	0.759	0.721
	0.784
1.25ng/ml	0.466	0.44	0.403
	0.415
0ng/ml	0.043	0.037	0
	0.031

Precision

We measured random samples of E1103Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	9.54	0.53	5.57
Intra-Assay	2	18	3.47	0.23	6.72
Intra-Assay	3	18	0.95	0.07	7.73

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102753	E1103Hu	48T	$320		Add to Cart
BHE12102753	E1103Hu	96T	$458		Add to Cart

Human Deoxyribonuclease-2-alpha - DNASE2A ELISA Kit

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