Human Deoxyribonuclease 1 - DNASE-1 ELISA Kit

Mfr.No.

E0876Hu

Description

This sandwich kit is for the accurate quantitative detection of human Deoxyribonuclease 1 (also known as DNase-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Deoxyribonuclease I;Deoxyribonuclease-1;DNase I;DNase-1;DNL1;Dornase alfa;DRNI

Reactivity

Human

Detection Range

0.5-100ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

31434

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.21ng/ml

Background

A deoxyribonuclease (DNase, for short) is an enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone, thus degrading DNA. Deoxyribonucleases are one type of nuclease, a generic term for enzymes capable of hydrolyzing phosphodiester bonds that link nucleotides. A wide variety of deoxyribonucleases are known, which differ in their substrate specificities, chemical mechanisms, and biological functions.

Gene ID	1773
UniProt ID	P24855
Antigen	DNase-1

Research Area

Others

Protocol

These standard curves of E0876Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64ng/ml	1.988	1.997	1.945
	2.006
32ng/ml	1.163	1.14	1.088
	1.116
16ng/ml	0.587	0.569	0.517
	0.55
8ng/ml	0.209	0.196	0.144
	0.182
4ng/ml	0.119	0.098	0.046
	0.077
0ng/ml	0.053	0.052	0
	0.051

Precision

We measured random samples of E0876Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	37.53	0.76	2.03
Intra-Assay	2	18	15.02	0.62	4.11
Intra-Assay	3	18	21.95	0.78	3.57

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102551	E0876Hu	48T	$320		Add to Cart
BHE12102551	E0876Hu	96T	$458		Add to Cart

Human Deoxyribonuclease 1 - DNASE-1 ELISA Kit

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