Human death-associated protein kinase (DAPK) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14181h

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

death-associated protein kinase 1, DAPK, DKFZp781I035

Recovery

The recovery of mouse F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	94-99
EDTA plasma (n=4)	91	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-89
1:2	Average %	109
	Range %	104-112
1:4	Average %	98
	Range %	94-102
1:8	Average %	92
	Range %	87-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P53355
Tissue Specificity	Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Applications	ELISA
Research Area	DAPK1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10502079	CSB-E14181h	10x96T	$7505	Add to Cart