Human Cytosolic Carboxypetidase 3

Mfr.No.

E4526Hu

Sensitivity

0.019ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

AGBL 3;AGBL3;ATP/GTP-binding protein-like 3;Cytosolic carboxypeptidase 3

Molecular Weight

116011

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4526Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.7	0.18	3.2
Intra-Assay	2	18	4	0.23	5.7
Intra-Assay	3	18	2.2	0.14	6.4

Background

Metallocarboxypeptidase that mediates both deglutamylation and deaspartylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates or polyaspartates from the carboxy-terminus of target proteins such as MYLK. Does not show detyrosinase or deglycylase activities from the carboxy-terminus of tubulin. (Isoform 2): Metallocarboxypeptidase that mediates tubulin deglutamylation.

Protocol

These standard curves of E4526Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.002	2.009	1.954
	2.015
6ng/ml	1.286	1.374	1.32
	1.462
3ng/ml	0.769	0.853	0.798
	0.936
1.5ng/ml	0.419	0.468	0.413
	0.517
0.75ng/ml	0.247	0.238	0.184
	0.228
0ng/ml	0.067	0.054	0
	0.042

Gene ID	340351
UniProt ID	Q8NEM8

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105842	E4526Hu	48T	$320		Add to Cart
BHE12105842	E4526Hu	96T	$458		Add to Cart

Human Cytosolic Carboxypetidase 3 - CCP3 ELISA Kit

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