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Home / ELISA Kit

Human Cytoplasmic aconitate hydratase (ACO1) ELISA Kit

SKU : BHE13703096

Aconitase 1 is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. The cytoplasmic IREBP interacts with the IREs of these mRNAs. The iron status of the cell determines the ability of IREBP to bind to an IRE through reversible oxidation-reduction of sulfhydryl groups that are critical for the high-affinity RNA-protein interaction. Thus, IREBP plays a central role in cellular iron homeostasis by regulating ferritin mRNA translation and TFRC mRNA stability

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Mfr.No.	KTE60880
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Cytoplasmic aconitate hydratase (ACO1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACO1.
Alternative Names	ACO1; ACONS; IREB1; IREBP; IREBP1; IRP1; OTTHUMP00000021177; aconitase 1; aconitate hydratase; citrate hydro-lyase; ferritin repressor protein; iron regulatory protein 1; iron-responsive element binding protein
Detection Method	Colorimetric
Kit Components	Human Cytoplasmic aconitate hydratase microplate Human Cytoplasmic aconitate hydratase standard Human Cytoplasmic aconitate hydratase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Cytoplasmic aconitate hydratase (ACO1) ELISA Kit has high sensitivity and excellent specificity for detection of Human ACO1. No significant cross-reactivity or interference between Human ACO1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Aconitase 1 is a cytosolic protein which binds to iron-responsive elements (IREs). IREs are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. The iron-induced binding to the IRE results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degrading transferrin receptor mRNA. The cytoplasmic IREBP interacts with the IREs of these mRNAs. The iron status of the cell determines the ability of IREBP to bind to an IRE through reversible oxidation-reduction of sulfhydryl groups that are critical for the high-affinity RNA-protein interaction. Thus, IREBP plays a central role in cellular iron homeostasis by regulating ferritin mRNA translation and TFRC mRNA stability
Notes	This Human Cytoplasmic aconitate hydratase (ACO1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACO1 in samples. An antibody specific for ACO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	48

Applications	ELISA
Applications Notes	This Human Cytoplasmic aconitate hydratase (ACO1) ELISA Kit employs a two-site sandwich ELISA to quantitate ACO1 in samples. An antibody specific for ACO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyACO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703096	KTE60880	48 T	$339		Add to Cart
BHE13703096	KTE60880	96 T	$589		Add to Cart
BHE13703096	KTE60880	96 Tx5	$2629		Add to Cart
BHE13703096	KTE60880	96 Tx50	$24569		Add to Cart

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