Human Cytokine Receptor-like Factor 2

Mfr.No.

E3177Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Cytokine receptor-like factor 2 (also known as CRLF2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CRLF 2;CRLF2;Cytokine receptor-like 2;Cytokine receptor-like factor 2;IL-XR;Thymic stromal lymphopoietin protein receptor;TSLP receptor

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

42013

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/ml

Background

Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2 (By similarity). Implicated in the development of the hematopoietic system.

Gene ID	64109
UniProt ID	Q9HC73
Antigen	CRLF2

Research Area

Immunology

Protocol

These standard curves of E3177Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.318	2.244	2.212
	2.171
6ng/ml	1.149	1.237	1.205
	1.325
3ng/ml	0.628	0.726	0.693
	0.823
1.5ng/ml	0.447	0.423	0.39
	0.399
0.75ng/ml	0.196	0.193	0.16
	0.189
0ng/ml	0.033	0.033	0
	0.032

Precision

We measured random samples of E3177Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3	0.17	5.7
Intra-Assay	2	18	8.6	0.23	2.6
Intra-Assay	3	18	4.4	0.23	5.3

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104706	E3177Hu	48T	$320		Add to Cart
BHE12104706	E3177Hu	96T	$458		Add to Cart

Human Cytokine Receptor-like Factor 2 - CRLF2 ELISA Kit

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