Human Cystatin-SN(CST1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL006088HU

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of mouse BALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	92-98
EDTA plasma (n=4)	92	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse BALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	99
	Range %	94-104
1:10	Average %	92
	Range %	88-98
1:20	Average %	84
	Range %	80-88
1:40	Average %	97
	Range %	92-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Cell culture supernatants, Tissue homogenates, Urine, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P01037
Tissue Specificity	Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in saliva, tears, urine and seminal fluid.

Applications	ELISA
Research Area	CST1

Storage	Store at 2-8°C. Please refer to protocol.

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