Human Cystatin M/Cystatin E/CST6 ELISA Kit PicoKine®

Mfr.No.

EK1965

Sensitivity

<10 pg/ml

Detection Range

46.9 pg/ml - 3,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	46.9	93.8	187.5	375	750	1500	3000
O.D.	0.020	0.058	0.098	0.192	0.392	0.775	1.429	2.010

Description

Human Cystatin M/CST6 ELISA Kit PicoKine® (96 Tests). Quantitate Human CST6 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cystatin-M; Cystatin-6; Cystatin-E; CST6

Immunogen

Expression system for standard: E.coli; Immunogen sequence: R29-M149

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Cystatin m/CST6

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	79	382	1415	83	409	1442
Standard deviation	4.90	17.19	80.66	4.07	23.72	90.85
CV (%)	6.2%	4.5%	5.7%	4.9%	5.8%	6.3%

Protein Function

Shows moderate inhibition of cathepsin B but is not active against cathepsin C.

Background

Cystatin-M is a protein that in humans is encoded by the CST6 gene. It is mapped to 11q13.1. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. This gene encodes a cystatin from the type 2 family, which is down-regulated in metastatic breast tumor cells as compared to primary tumor cells. Loss of expression is likely associated with the progression of a primary tumor to a metastatic phenotype.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	79	79	75	76	77	1.78	2.3%
Sample 2	382	396	405	372	388	12.67	3.2%
Sample 3	1415	1318	1387	1330	1362	39.97	2.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1474
Gene Name	CST6
UniProt ID	Q15828
Tissue Specificity	Restricted to the stratum granulosum of normal skin, the stratum granulosum/spinosum of psoriatic skin, and the secretory coils of eccrine sweat glands. Low expression levels are found in the nasal cavity.

Applications	ELISA
Research Area	Cell Biology,Protease Inhibitors,Proteolysis/Ubiquitin

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21001472	EK1965	96 wells/kit	$499		Add to Cart

Human Cystatin M/Cystatin E/CST6 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Cystatin M/Cystatin E/CST6 ELISA Kit PicoKine®

Rate it now..!!