Human Cystatin C/CST3 PicoKine® Quick ELISA Kit

Mfr.No.

FEK0678

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312.5	625	1250	2500	5000	10000	20000
O.D.	0.032	0.118	0.184	0.333	0.593	1.041	1.706	2.078

Description

Human Cystatin C/CST3 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CST3 in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cystatin-C;Cystatin-3;Gamma-trace;Neuroendocrine basic polypeptide;Post-gamma-globulin;CST3;

Molecular Weight

15799 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-A146

Kit Components

Description Quantity Volume
Pre-coated 96-well Strip Microplate 1 12 strips of 8 wells
Standard 2
HRP-Linked Antibody 1 6ml
Sample Diluent 1 15ml
TBS-T Wash Buffer (25x) 1 12ml
Color Developing Reagent (TMB) 1 10ml
Stop Solution 1 10ml
Adhesive Plate Sealers 2 Piece

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant Human CST3

Subcellular Localization

Secreted .

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	695	2907	11478	712	3114	12116
Standard deviation	45.87	212.21	791.98	56.24	255.34	1054.09
CV (%)	6.6%	7.3%	6.9%	7.9%	8.2%	8.7%

Protein Function

As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity.

Background

Cystatin C or cystatin 3 (formerly gamma trace, post-gamma-globulin or neuroendocrine basic polypeptide), a protein encoded by the CST3 gene, was originally described as a constituent of normal cerebrospinal fluid (CSF) and of urine from patients with renal failure.1 Cystatin 3 has a low molecular weight (approximately 13.3 kilodaltons), and it is removed from the bloodstream by glomerular filtration in the kidneys. In humans, all cells with a nucleus (cell core containing the DNA) produce cystatin C as a chain of 120 amino acids. It is found in virtually all tissues and bodily fluids. Cystatin C, which belongs to the type II cystatin gene family, is a potent inhibitor of lysosomal proteinases2 (enzymes from a special subunit of the cell that break down proteins) and probably one of the most important extracellular inhibitors of cysteine proteases3 (it prevents the breakdown of proteins outside the cell by a specific type of protein degrading enzymes). Moreover, cystatin C is involved in network reorganization in the epileptic dentate gyrus.4

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	695	651	676	688	677	14.89	2.2%
Sample 2	2907	2015	2878	2769	2642	73.97	2.8%
Sample 3	11478	9967	8907	10146	10124	546.69	5.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

Gene ID	1471
Gene Name	CST3
UniProt ID	P01034
Protein Name	Cystatin-C
Tissue Specificity	Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. .

Applications	ELISA
Research Area	Apoptosis,Atherosclerosis,Cardiovascular,Caspases,Cell Biology,Cell Type Markers,Intracellular,Neural Stem Cells,Stem Cells,Tags & Cell Markers,Tumor Associated

Storage	Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000215	FEK0678	96 wells/kit	$499		Add to Cart

Human Cystatin C/CST3 PicoKine® Quick ELISA Kit

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