Matrices listed below were spiked with certain level of CYP3A5 and the recovery rates were calculated by comparing the measured value to the expected amount of CYP3A5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
90
EDTA plasma(n=5)
90-103
99
heparin plasma(n=5)
92-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CYP3A5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CYP3A5 ELISA Kit allows for the in vitro quantitative determination of CYP3A5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human CYP3A5(Cytochrome P450 3A5) ELISA Kit