Human CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1) CLIA Kit

Mfr.No.

E-CL-H0307

Sensitivity

18.75 pg/mL

Detection Range

31.25-2000 pg/mL

Reactivity

Human

Assay Duration

3.5h

Assay Type

Sandwich

Description

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CYP24A1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CYP24A1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CYP24A1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CYP24A1. You can calculate the concentration of Human CYP24A1 in the samples by comparing the RLU value of the samples to the standard curve.

Alternative Names

CP24 ; CYP24 ; HCAI ; P450-CC24 ; 1 ; 25-dihydroxyvitamin D3 24-hydroxylase ; mitochondrial

Detection Method

Chemiluminescence

Recovery

80%-120%

Linearity

Samples were spiked with high concentrations of Human CYP24A1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	91-105	89-105	91-101
1:2	Average (%)	98	96	96
1:4	Range (%)	87-98	88-101	95-109
1:4	Average (%)	93	95	102
1:8	Range (%)	92-107	98-114	92-105
1:8	Average (%)	99	105	98
1:16	Range (%)	86-98	101-118	93-104
1:16	Average (%)	92	109	99

Specificity

This kit recognizes Human CYP24A1 in samples.No significant cross-reactivity or interference between Human CYP24A1 and analogues was observed

Sample Type

Cell lysates, Tissue homogenates, Other biological fluids

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Precision

Both intra-CV and inter-CV are < 10%.

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Human CYP24A1 concentrations in Tissue homogenates,cell lysates and other biological fluids.

UniProt ID	Q07973

Applications	CLIA
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Storage	2-8°C/-20°C,6 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10300269	E-CL-H0307	96T	$682		Add to Cart
BHE10300269	E-CL-H0307	48T	$546		Add to Cart

Human CYP24A1 (Cytochrome P450, family 24, subfamily A, polypeptide 1) CLIA Kit

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